Anti-CD31 (Human) LIMAAbody® #10001
Alpaca anti-human CD31 VHH is an in-house developed and validated nanobody (single-domain antibody) binding to human CD31 (PECAM-1) highly suitabe for tissue staining.
Delivery time: seven workdays.
Alpaca anti-human CD31 VHH is an in-house developed and validated nanobody (single-domain antibody) binding to human CD31 (PECAM-1) highly suitabe for tissue staining.
Delivery time: seven workdays.
Alpaca anti-human CD31 VHH is an in-house developed and validated nanobody (single-domain antibody) binding to human CD31 (PECAM-1) highly suitabe for tissue staining.
Delivery time: seven workdays.
Documentation:
Product Information:
Applications tested
Immunofluorescence staining, wholemount tissue staining.
Specificity/Target
Human CD31 (PECAM-1). Validated on human tissue samples and cell lines.
Type
Nanobody, single-domain antibody, VHH
Clone
Polyclonal: 2MAC4, 2PEC47, 3MAC24, 3MAC69, 3MAC82, 3PEC92
Reactivity
Human. Does not show cross-reactivity with murine CD31 based on staining of tissue samples and cell lines.
Immunogen
Recombinant protein containing the extracellular domain of CD31 protein.
Class
Recombinant, produced in E.coli.
Host
Alpaca
Sensitivity
Endogenous protein expression.
Conjugate
Unconjugated.
Suitable for conjugation of fluorescent dyes, biotin, beads, surfaces etc. via NHS ester chemistry.
VHH protein tag
C-terminal (6x)His-Tag
Purity
Affinity-purified antibody fragment.
Molecular Weight
2MAC4: 14,7 kDa
2PEC47: 16,7 kDa
3MAC24: 15,4 kDa
3MAC69: 15,3 kDa
3MAC82: 16,6 kDa
3PEC92: 14,1 kDa
Form
Buffered solution.
Storage buffer
Phosphate buffered saline (PBS), pH=7,4; Preservative: 0.03% Sodium azide.
Storage condition
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 6 months. Do not freeze.
Product Usage Information
For optimal immunofluorescence staining results, use nanobody at concentrations between 10-30µg/ml in combination with recommended LIMAA reagents and protocols.
Application and Dilution
Immunofluorescence (frozen sections): 10 µg/ml
Immunofluorescence (cell culture): 5 µg/ml
lmmunofluorescence (wholemount tissue staining): 20 µg/ml
Background
CD31, also known as PECAM-1, is an adhesion molecule, which is expressed on endothelial cells at intercellular junctions and various T cell subsets. It is also found to be expressed on platelets, as well as most other leukocytes including monocytes and neutrophils. CD31 facilitates homotypic binding to itself, as well as heterotypic binding to the leukocyte integrins. CD31 plays a crucial role in facilitating the transendothelial migration of leukocytes through the intercellular junctions of vascular endothelial cells.
Trademarks and Patents
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