Anti-CD31 (Mouse) LIMAAbody® #10002

Product Information:

Applications tested
Immunofluorescence staining, wholemount tissue staining.

Specificity/Target
Mouse CD31 (PECAM-1). Validated on mouse tissue samples

Type
Nanobody, single-domain antibody, VHH

Clone
Polyclonal: 3ELC120, 3ELC140, 3ELC105, 3ELC143

Reactivity
Mouse. Reactivity based on staining of tissue samples and cell lines.

Immunogen
Recombinant protein containing the extracellular domain of murine CD31 protein.

Class
Recombinant, produced in E.coli.

Host
Alpaca

Sensitivity
Endogenous protein expression.

Conjugate
Unconjugated.
Suitable for conjugation of fluorescent dyes, biotin, beads, surfaces etc. via NHS ester chemistry.

VHH protein tag
C-terminal (6x)His-Tag

Purity
Affinity-purified antibody fragment.

Molecular Weight
3ELC120: 15 kDa
3ELC140: 15 kDa
3ELC105: 15 kDa
3ELC143: 15 kDa

Form
Buffered solution.

Storage buffer
Phosphate buffered saline (PBS), pH=7,4; Preservative: 0.03% Sodium azide.

Storage condition
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 6 months. Do not freeze.

Product Usage Information
For optimal immunofluorescence staining results, use nanobody at concentrations between 10-30µg/ml in combination with recommended LIMAA reagents and protocols.

Application and Dilution
Immunofluorescence (frozen sections): 10 µg/ml
Immunofluorescence (cell culture): 1 µg/ml
lmmunofluorescence (wholemount tissue staining): 20 µg/ml

Background
CD31, also known as PECAM-1, is an adhesion molecule and is expressed on endothelial cells at intercellular junctions and various T cell subsets. It shows altered expression levels in arterial, venous and lymphatic vessels. It is also found to a lesser extent on platelets, as well as most other leukocytes including monocytes and neutrophils. CD31 facilitates homotypic binding to itself, as well as heterotypic binding to the leukocyte integrin alpha V beta III. CD31 plays a crucial role in facilitating the transendothelial migration of leukocytes through the intercellular junctions of vascular endothelial cells. It is one of key regulatory molecules in vascular system.

Trademarks and Patents
LIMAAbody® is a trademark of LIMAA Technologies GmbH.
ATTO is a registered trademark of ATTO-TEC GmbH.
All other trademarks are the property of their respective owners.

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