Anti-CD56 (Human) LIMAAbody® #10004

Documentation:

• Download Datasheet

• Download MSDS

Product Information:

Applications tested
Immunofluorescence staining, wholemount tissue staining.

Specificity/Target
Human CD56. Validated on human tissue samples and cell lines. 

Type
Nanobody, single-domain antibody, VHH

Clone
Polyclonal: 3FIF12, 2FIF67

Reactivity
Human. Reactivity based on IF and IHC stainings of tissue samples and cell lines.

Immunogen
Recombinant protein containing the extracellular domain of human CD56 protein.

Class
Recombinant, produced in E.coli.

Host
Alpaca

Sensitivity
Endogenous protein expression.

Conjugate
Unconjugated.
Suitable for conjugation of fluorescent dyes, biotin, beads, surfaces etc. via NHS ester chemistry.

VHH protein tag
C-terminal (6x)His-Tag

Purity
Affinity-purified antibody fragment.

Molecular Weight
3FIF12: 15 kDa
2FIF67: 15 kDa

Form
Buffered solution.

Storage buffer
Phosphate buffered saline (PBS), pH=7,4; Preservative: 0.03% Sodium azide.

Storage condition
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 6 months. Do not freeze.

Product Usage Information
For optimal immunofluorescence staining results, use nanobody at concentrations between 10-30µg/ml in combination with recommended LIMAA reagents and protocols.

Application and Dilution
Immunofluorescence (frozen sections): 10 µg/ml
Immunofluorescence (cell culture): 1 µg/ml
lmmunofluorescence (wholemount tissue staining): 20 µg/ml

Background
CD56, also known as Neural Cell Adhesion Molecule (NCAM), is a glycoprotein expressed on neurons, glial cells, skeletal muscle, NK cells, and some T-cells. It belongs to the immunoglobulin superfamily, with five immunoglobulin-like domains and two fibronectin type III repeats, enabling cell adhesion and signaling.
In the nervous system, NCAM is vital for neurodevelopment, synaptic plasticity, and response to injury. In the immune system, it influences NK cell function and development. Abnormal NCAM expression is linked to neurodevelopmental and neurodegenerative disorders, as well as cancers, making it a crucial biomarker and therapeutic target.

Trademarks and Patents
LIMAAbody® is a trademark of LIMAA Technologies GmbH.
ATTO is a registered trademark of ATTO-TEC GmbH.
All other trademarks are the property of their respective owners.

Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of LIMAA, the following terms apply to Products provided by LIMAA, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of LIMAA, are rejected and are of no force or effect.

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